FertiCult PVP 10%

Ready-to-use viscous medium containing polyvinylpyrrolidone (PVP) used for sperm preparation for Intracytoplasmic Sperm Injection (ICSI). These procedures require the capture of individual sperm cells in a glass pipette for injection into the oocyte and this is facilitated by first immobilizing the sperm by placing them in a viscous medium FertiCult™ 10% PVP prior to nicking the tail to immobilize sperm completely.

• Packaged as 5 x 0,2 ml bottles or as 1 x 1 ml bottle.
• 10% (w/v) dialysed Ph. Eur. grade polyvinylpyrrolidone (PVP) in FertiCultTM Flushing medium.
• FertiCultTM Flushing medium is an aqueous solution containing HEPES, bicarbonate, physiologic salts, glucose, lactate, pyruvate and human serum albumin (derived from human blood plasma).
• Performance: pH 7.20-7.90 ; Osmolality: 280-340 mOsm/kg ; Viscosity: >170 cP at 25°C ; HSA conc.: 3.2-4.8 g/l.

Components include: Ultrapure water, PVP, Sodium Chloride, HEPES, Human Serum Albumin (HSA), Sodium lactate, Glucose monohydrate, Sodium hydrogen carbonate, Potassium chloride, Calcium chloride dihydrate, Sodium dihydrogen phosphate dihydrate, Magnesium sulphate heptahydrate, Sodium pyruvate.